- Liftover to change your genomic coordinates e.g. from mm9 to mm10
- The Picard tool suite has been updated and expanded. For example, the following tools are now available: Downsample SAM/BAM, CleanSam, AddCommentsToBam, ValidateSamFile, EstimateLibrarySizeComplexity
- The full Bedtools suite is now available. Tools incl. SlopBed, ClosestBed and WindowBed
- Filter BAM, which allows you to filter multi-mapping reads obtained in PE experiments. Tool written by Charles Girardot.
- Update/new regional variation tools: 
- Fetch Indels from 3-way alignments
- Indel Realigner - perform local realignment
- Estimate Indel Rates for 3-way alignments
- Delete Overlapping Indels from a chromosome indels file
- Fetch Indels from pairwise alignments
- Cuffcompare, Cufflinks, Cuffdiff and Cuffmerge run version 2.2.1 now
- NFS Transfer has been updated
- Compute_motif_frequencies_for_all_motifs and compute_motifs_frequency are back
- The converters: bigwig_to_wig and bam_to_bigwig have been added
- The deeptools package updated to version 1.5.9.1
- Added CEAS to characterize genome-wide protein-DNA interaction patterns from ChIP-chip and ChIP-Seq